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Postdoctoral Research (2023-2025)

Department of Plant Science, McGill University

Project Title: Plant-derived biosynergists to enhance pesticide efficacy

Supervisor: Dr. Mehran Dastmalchi

                       Dr. Christopher J. Thibodeaux (Co-supervisor)

  • Developed reproducible RNA-seq pipelines (STAR, featureCounts, DESeq2) and proteomics workflows.

  • Applied Python and R scripts for data preprocessing, quality control, and statistical modeling.

  • Integrated multi-omics data (RNA-seq, proteomics, metabolomics) using machine learning approaches for candidate prioritization.

  • Developed enzyme activity assays to evaluate protein function and ligand interactions.

  • Standardized and applied Differential Scanning Fluorimetry (DSF) and Hydrogen Deuterium Exchange Mass Spectrometry (HDX-MS) assays to study protein ligand stability and conformational changes.

  • Manuscripts in preparation for submission!

Doctoral Research (2017-2023)

Department of Plant Science, McGill University

Thesis Title: Deciphering the molecular mechanisms underlying the interaction of Bacillus velezensis strain B26 with the model plant Brachypodium distachyon.

Supervisor: Dr Suha Jabaji​

  • Investigated the molecular mechanisms underlying beneficial plant–microbe interactions, with a focus on plant growth–promoting bacteria Bacillus velezensis strain B26 and the model grass Brachypodium distachyon.

  • Examined how microbial colonization influences plant metabolism, signaling pathways, and growth promotion.

  • Applied integrative transcriptomic and metabolomic approaches to identify key organic acids and associated genes involved in rhizobacterial recruitment.

  • Performed GC–MS–based metabolite profiling and phytohormone analyses to assess microbial effects on plant metabolic and hormonal pathways.

  • Conducted detailed gene expression analyses of phytohormone biosynthesis and regulatory pathways, providing mechanistic insights into plant growth promotion.

  • Applied dual RNA-seq analysis and mutant characterization in Brachypodium, including functional studies of flowering-time genes.​

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Senior Research Fellow (2015- 2017)

Department of Plant Breeding and Genetics, Punjab Agricultural University, India.

Project Title: Broadening the genetic base of mustard (Brassica juncea) through alien introgressions and Germplasm enhancement

Supervisor: Dr. S.S Banga

  • Designed and executed large-scale genomics experiments, including SNP discovery, genome-wide association studies (GWAS), and population-level genetic analyses.

  • Performed comprehensive RNA-seq workflows for Brassica species, including read processing, genome assembly, and differential expression analysis.

  • Developed and optimized bioinformatics pipelines to support high-throughput sequencing projects and downstream statistical analyses.

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© Meha Sharma

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